Chemical synthesis of the novel Ca2+ messenger NAADP

The first total chemical synthesis of nicotinamide adenine dinucleotide phosphate (P-NADP, 2) as a single isomer was achieved. Thin was subsequently converted into the important second messenger nicotinic acid adenine dinucleotide phosphate (P-NAADP) 1 and the identity of this material confirmed by biological evaluation. This flexible synthetic route offers new opportunities for the generation of NAADP I analogues that cannot be generated directly from NADP 2 or mainly enzymatic methods.