期刊
FRONTIERS IN BIOSCIENCE-LANDMARK
卷 10, 期 -, 页码 47-53出版社
FRONTIERS IN BIOSCIENCE INC
DOI: 10.2741/1505
关键词
peroxynitrite; glucose transport; PI3-K; PKB; wortmannin; 3T3-L1 adipocytes
Peroxynitrite, the product of the reaction between (NO)-N-center dot and O-2(center dot-), is a strong oxidant and nitrating molecule, and it has been recently considered as a component of some important signaling pathways. Herein, we report the effect of peroxynitrite on glucose uptake in 3T3-L1 adipocytes. Peroxynitrite stimulated glucose uptake and this effect was inhibited by citochalasin B, indicating the participation of facilitated GLUT transporters. Peroxynitrite- induced glucose uptake was not related to intracellular ATP, nor to external or internal calcium, but it was inhibited by the phosphatidylinositol 3-kinase (PI3-K) inhibitor, wortmannin. Additionally, we also found that peroxynitrite did not activate the insulin receptor nor the PI3-K downstream signaling protein kinase B (PKB/Akt). The dose-dependent inhibitory action of wortmannin suggests that peroxynitrite activates glucose transport without affecting GLUT transporters translocation.
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