Chemometrically assisted development of IP-RP-HPLC and spectrophotometric methods for the identification and determination of synthetic dyes in commercial soft drinks

Two independent methods were developed to identify and determinate synthetic dyes and to follow their degradation processes. The IP-RP-HPLC method was optimised by the experimental design technique and the results obtained were compared with ones obtained by direct/derivative spectrophotometry. The dyes considered in this study that are representative of soft drink components, are: Tartrazinc (El 02), Quinoline Yellow (E 104), Sunset Yellow (E I 10), Carmoisine (E 122), Amaranth (E 123), New Coccine (El 24), Patent Blue Violet (E 13 1), and Brillant Blue FCF (E 133).