期刊
JOURNAL OF LIPID RESEARCH
卷 46, 期 1, 页码 21-26出版社
ELSEVIER
DOI: 10.1194/jlr.C400004-JLR200
关键词
free phytosterols; phytosoterol esters; liquid chromatography; plant sterols
A novel analytical platform based on liquid chromatography and tandem mass spectrometry using atmospheric pressure photoionization was applied for the simultaneous quantification of free and esterified P-sitosterol, campesterol, brassicasterol, and stigmasterol. The total time for sample pretreatment and analysis could be reduced from similar to3 h [gas chromatography-mass spectrometry (GC-MS)] to 15 min. The detection limits of the different phytosterols; ranged between 0.25 and 0.68 mug/l. Linear ranges were between 1 and 1,000 mug/l. The within-run and between-rim variabilities ranged between 1.4% and 9.9%. The analytical sensitivity was at least 150-fold higher compared with GC-MS. Our new method allows a rapid and simultaneous determination of free and esterified phytosterols in serum.
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