期刊
NEUROPHARMACOLOGY
卷 51, 期 3, 页码 426-437出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuropharm.2006.03.031
关键词
thermoregulation; excitatory amino acid; energy expenditure; sympathetic nerve activity
资金
- NIDDK NIH HHS [DK20378, DK57838] Funding Source: Medline
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK020378, R01DK057838] Funding Source: NIH RePORTER
Disinhibition of DMH neurons with the GABA(A) receptor antagonist, bicuculline, increases heart rate (HR) and augments both brown adipose tissue sympathetic nerve activity (BAT SNA) and renal SNA (RSNA) contributing to the evoked increases in BAT thermogenesis and arterial pressure (AP). We determined the role of glutamate receptor activation in the rostral raphe pailidus (RPa) in mediating the sympathoexcitatory responses in HR, BAT SNA and RSNA following disinhibition of DMH neurons in urethane/chloralose anesthetized, artificially ventilated rats. Microinjections of either the selective NMDA receptor agonist, NMDA, or the selective non-NMDA receptor agonist, kainic acid (KA), into the RPa produced increases in BAT SNA (peak: +502% and +408% of control, respectively) and BAT temperature (peak: +0.6 degrees C and +1.0 degrees C) accompanied by rises in HR (peak: +38 and +63 bpm), RSNA (peak: +57% and +58% of control) and MAP (peak: +12 and 15 mmHg). These responses were reversed by subsequent microinjection into RPa of the respective selective glutamate receptor antagonists, AP5 and CNQX. Microinjections of the non-selective glutamate receptor antagonist, kynurenic acid (Kyn), the NMDA receptor antagonist, AP5, or the non-NMDA receptor antagonist, CNQX, were effective in reversing the increases in BAT SNA (for Kyn, from peak of +419% of control to +9% of control) and BAT temperature, but not those in HR, MAP or RSNA (for Kyn, from peak of +143% of control to +124% of control) evoked by unilateral microinjection of bicuculline into the DMH. These results indicate that both NMDA and non-NMDA glutamate receptors in the RPa play a significant role in mediating the excitatory synaptic transmission producing the activation of BAT thermogenesis following disinhibition of DMH neurons. Glutamate receptors in the RPa may not be important for transmitting cardiovascular responses induced by activation of the DMH neurons. (c) 2006 Elsevier Ltd. All rights reserved.
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