期刊
CHROMATOGRAPHIA
卷 63, 期 11-12, 页码 605-608出版社
SPRINGER HEIDELBERG
DOI: 10.1365/s10337-006-0807-9
关键词
column liquid chromatography; diode-array and fluorescence detection; fluoroquinolones; gatifloxacin in semen
The objective of this work was to develop an analytical HPLC method, using DAD and fluorescence detection, for determination of gatifloxacin in semen. A reversed-phase column was used with 90:10 water-acetonitrile, containing 10 mM TBA and 25 mM citric acid, as mobile phase. Semen was deproteinized with acetonitrile. Recovery was 95 +/- 10%. The limits of quantification by DAD and fluorescence were 2.3 and 0.03 mu g.mL(-1) respectively, with RSD of 3.4% for DAD and 2.8% far fluorescence. The method with fluorescence detection was used for quantification of gatifloxacin in the semen of patients under treatment.
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