Determination of gatifloxacin in semen by HPLC with diode-array and fluorescence detection

The objective of this work was to develop an analytical HPLC method, using DAD and fluorescence detection, for determination of gatifloxacin in semen. A reversed-phase column was used with 90:10 water-acetonitrile, containing 10 mM TBA and 25 mM citric acid, as mobile phase. Semen was deproteinized with acetonitrile. Recovery was 95 +/- 10%. The limits of quantification by DAD and fluorescence were 2.3 and 0.03 mu g.mL(-1) respectively, with RSD of 3.4% for DAD and 2.8% far fluorescence. The method with fluorescence detection was used for quantification of gatifloxacin in the semen of patients under treatment.