4.3 Article

Secretion of human epidermal growth factor by Corynebacterium glutamicum

期刊

LETTERS IN APPLIED MICROBIOLOGY
卷 42, 期 1, 页码 66-70

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WILEY
DOI: 10.1111/j.1472-765X.2005.01802.x

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active protein; Corynebacterium glutamicum; human epidermal growth factor; protein secretion

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Aims: To examine the secretion of human epidermal growth factor (hEGF) by Corynebacterium glutamicum. Methods and Results: We recently showed that a novel protein-secretion system in C. glutamicum could produce Streptomyces mobaraensis transglutaminase. In the present study, the industrially important protein hEGF was secreted into the culture medium in a fully active form by C. glutamicum and accumulated at a rate of up to 156 mg l(-1) day(-1). Conclusions: These results demonstrated that the hEGF protein could be secreted in an active form by C. glutamicum. Significance and Impact of the Study: Our data confirmed that the pharmaceutically important human protein hEGF could be efficiently secreted in an active form by the C. glutamicum protein-expression system. Moreover, we demonstrated that this bacterium has potential as a host for the industrial-scale production of human proteins.

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