期刊
ANALYTICAL LETTERS
卷 39, 期 11, 页码 2227-2233出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/00032710600751016
关键词
creatine; creatinine; amperometric biosensors; creatininase; creatinase; sorcosine oxidase
In order to determine creatine and creatinine, amperometric diamond paste biosensors were proposed. A bienzymatic biosensor based on creatinase and sarcosine oxidase was used for the assay of creatine and a trienzymatic biosensor based on creatinase, sarcosine oxidase, and creatininase was proposed for the assay of creatinine. The linear concentration ranges are of fmol/L magnitude order, with very low limits of detection. The biosensors proved to be highly reliable for determination of creatine and creatinine as raw materials in pharmaceutical formulations as well as in serum samples.
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