Simultaneous determination and identification of furazolidone, furaltadone, nitrofurazone, and nitrovin in feeds by HPLC and LC-MS

An HPLC and LC-MS method for simultaneous determination of furazolidone, furaltadone, nitrofurazone, and nitrovin in feeds was developed. Samples were extracted with a mixture of acetonitrile and methanol with ammonia solution, and further clean-up was achieved with solid-phase extraction (SPE). Separation and quantitation of the analyte were developed with an acetonitrile-water gradient program and UV absorbance at 365 nm. LC-MS was in an electrospray positive ion mode. The limits of detection were 0.1 mg/kg for furazolidone and furaltadone, and 0.2 mg/kg for nitrofurazone and nitrovin; the limits of quantitation in feeds were 1 mg/kg for furazolidone and furaltadone, and 2 mg/kg for nitrofurazone and nitrovin.