期刊
CANCER RESEARCH
卷 73, 期 11, 页码 3297-3305出版社
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/0008-5472.CAN-12-3970
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资金
- Prostate Cancer Program of the Department of Defense [W81XWH-09-1-0724]
Despite the progress made in the early detection and treatment of prostate adenocarcinoma, the metastatic lesions from this tumor are incurable. We used genome-wide expression analysis of human prostate cancer cells with different metastatic behavior in animal models to reveal that bone-tropic phenotypes upregulate three genes encoding for the cytokine interleukin-1 beta (IL-1 beta), the chemokine CXCL6 (GCP-2), and the protease inhibitor elafin (PI3). The Oncomine database revealed that these three genes are significantly upregulated in human prostate cancer versus normal tissue and correlate with Gleason scores >= 7. This correlation was further validated for IL-1b by immunodetection in prostate tissue arrays. Our study also shows that the exogenous overexpression of IL-1 beta in nonmetastatic cancer cells promotes their growth into large skeletal lesions in mice, whereas its knockdown significantly impairs the bone progression of highly metastatic cells. In addition, IL-1 beta secreted by metastatic cells induced the overexpression of COX-2 (PTGS2) in human bone mesenchymal cells treated with conditioned media from bone metastatic prostate cancer cells. Finally, we inspected human tissue specimens from skeletal metastases and detected prostate cancer cells positive for both IL-1 beta and synaptophysin while concurrently lacking prostate-specific antigen (PSA, KLK3) expression. Collectively, these findings indicate that IL-1 beta supports the skeletal colonization and metastatic progression of prostate cancer cells with an acquired neuroendocrine phenotype. (C) 2013 AACR.
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