期刊
ARCHIVES OF PHARMACAL RESEARCH
卷 29, 期 9, 页码 757-761出版社
PHARMACEUTICAL SOCIETY KOREA
DOI: 10.1007/BF02974076
关键词
Coptis japonica; Ranunculaceae; protoberberine alkaloid; 8-oxo-epiberberine; multidrug resistance
Six protoberberine alkaloids were isolated from the chloroform layer of the rhizome of Coptis japonica Makino (Ranunculaceae). The structures of the isolated compounds were determined to be 6-([1,3]dioxolo[4,5-g]isoquinoline-5-carbonyl)-2,3-dimethoxy-benzoic acid methyl ester (1), oxyberberine (2), 8-oxo-epiberberine (3), 8-oxocoptisine (4), berberine (5) and palmatine (6) by physicochemical and spectroscopic methods. The compound 3 (8-oxo-epiberberine) was first isolated from natural sources. The compounds were tested for cytotoxicity against five tumor cell lines in vitro by SRB method, and also tested for the MDR reversal activities. Compound 4 was of significant P-gp MDR inhibition activity with ED50 value 0.018 mu g/mL in MES-SA/DX5 cell and 0.0005 mu g/mL in HCT15 cell, respectively.
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