期刊
BIOMEDICAL CHROMATOGRAPHY
卷 20, 期 6-7, 页码 612-621出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/bmc.656
关键词
porphyrias; porphyrin excretion patterns; HPLC/electrospray ionization tandem mass spectrometry
A high-resolution high-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry method is described for the analysis of porphyrins in blood, urine and faeces. The gradient elution reversed-phase HPLC system using acetonitrile-methanol-1 M ammonium acetate/acetic acid buffer (pH 5.16) as gradient solvent mixtures was able to separate all porphyrin metabolites, including the type I and type III isomers of uroporphyrin, hepta-, hexa- and penta-carboxylic acid porphyrins and coproporphyrin. The porphyrins were positively identified by the protonated molecules [M+H](+) and further characterized by tandem mass spectrometric analysis with each porphyrin giving a characteristic collision-induced dissociation product ion spectrum. The mass chromatograms obtained by HPLC/ESI MS are useful for the differential diagnosis of the porphyrias, since each type of porphyria has a typical porphyrin excretion pattern. Copyright (c) 2006 John Wiley & Sons, Ltd.
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