期刊
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
卷 5, 期 6, 页码 621-628出版社
SPRINGERNATURE
DOI: 10.1039/b516533d
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资金
- MRC [MC_U117532185] Funding Source: UKRI
- Medical Research Council [MC_U117532185] Funding Source: researchfish
Using 1-(2-nitrophenyl)ethyl sulfate (caged sulfate) as a photoactivatable caged proton, we could induce complete acid unfolding of myoglobin with a single nanosecond laser pulse. This was possible because of the high (approximate to mM) concentration of protons released by the photolabile compound. The ability of the compound to produce a large pH jump arises because the other photoproducts (2-nitrosoacetophenone and sulfate ion) do not buffer the released protons. The complete time course of the unfolding kinetics, spanning a range from milliseconds to several seconds, could be accurately reproduced by monitoring absorbance changes in the visible spectrum at 633 nm.
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