期刊
MOLECULAR BIOSYSTEMS
卷 2, 期 1, 页码 25-35出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b514349g
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资金
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [P01DK058398] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM058175] Funding Source: NIH RePORTER
- NHLBI NIH HHS [N01-HV-28185] Funding Source: Medline
- NIDDK NIH HHS [P01-DK58398] Funding Source: Medline
- NIGMS NIH HHS [R01 GM58175] Funding Source: Medline
Many methods have been published by which combinatorial libraries may be screened for compounds capable of manipulating the function(s) of a target protein. One of the simplest approaches is to identify compounds in a library that bind the protein of interest, since these binding events usually occur on functionally important surfaces of the protein. These protein-binding compounds could also be of utility as protein capture agents in the construction of protein-detecting microarrays or related analytical devices and as reagents for the affinity purification of proteins from complex mixtures. In this article, we provide optimized methods for screening libraries of molecules displayed on the beads on which they were synthesized. This is a particularly convenient format for library screening for laboratories with limited budgets and modest robotics capabilities.
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