期刊
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
卷 17, 期 1-2, 页码 187-197出版社
TAYLOR & FRANCIS LTD
DOI: 10.1163/156856206774879090
关键词
heparin; hydrogel; controlled release; vascular endothelial growth factor; angiogenesis
资金
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R24HL064387] Funding Source: NIH RePORTER
- NHLBI NIH HHS [R24-HL64387] Funding Source: Medline
An affinity-based controlled release system for growth factors having heparin-binding domains was prepared using a cross-linked heparin gel. The heparin gel was made by reacting hydrazide-functionalized heparin (Hep-ADH) with the N-hydroxysuccinimidyl ester of poly(ethylene glycol)-bis-butanoic acid (SBA-PEG-SBA). The degree of cross-linking could be controlled by defining the stoichiometry of hydrazide modification and the PEG cross-linker addition. The release of vascular endothelial growth factor (VEGF) was characterized as a heparin-binding growth factor. VEGF was directly injected into the heparin gel and the loaded VEGF displayed a slow, controlled release over 3 weeks with little initial burst phase. The biological activity of the released VEGF was measured with a proliferation assay utilizing human umbilical vein endothelial cells. The released VEGF maintained its biological activity at all time points investigated. The heparin gel with loaded VEGF was implanted sub-cutaneously in the dorsal region of mice. A significantly increased density of the endothelial cell marker platelet endothelial adhesion molecule (PECAM-1) was observed in histological specimens of the tissues surrounding the implanted gel.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据