期刊
CYTOTECHNOLOGY
卷 51, 期 2, 页码 45-50出版社
SPRINGER
DOI: 10.1007/s10616-006-9013-8
关键词
cell lines; cross-contamination; fingerprinting; isoenzyme analysis; short tandem repeat; variable number tandem repeat
Cell line cross-contamination is a phenomenon that arises as a result of the continuous cell line culture. It has been estimated that around 20% of the cell lines are misidentified, therefore it is necessary to carry out quality control tests for the detection of this issue. Since cell line cross-contamination discovery, different methods have been applied, such as isoenzyme analysis for inter-species cross-contamination; HLA typing, and DNA fingerprinting using short tandem repeat and a variable number of tandem repeat for intra-species cross-contamination. The cell banks in this sense represent the organizations responsible for guaranteeing the authenticity of cell lines for future research and clinical uses.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据