期刊
INTERNATIONAL JOURNAL OF NANOMEDICINE
卷 1, 期 1, 页码 51-57出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/nano.2006.1.1.51
关键词
gold nanoparticles; hetero-bifunctional poly(ethylene glycol) spacer; surface functionalization; cellular trafficking; cytotoxicity
资金
- NATIONAL CANCER INSTITUTE [R01CA095522] Funding Source: NIH RePORTER
- NCI NIH HHS [R01 CA095522, R01-CA095522, R01 CA095522-03] Funding Source: Medline
For the development of surface-functionalized gold nanoparticles as cellular probes and delivery agents, we have synthesized hetero-bifunctional poly(ethylene glycol) (PEG, MW 1500) having a thiol group on one terminus and a reactive functional group on the other for use as a flexible spacer. Comarin, a model fluorescent dye, was conjugated to one end of the PEG spacer and gold nanoparticles were modified with coumarin-PEG-thiol. Surface attachment of coumarin through the PEG spacer decreased the fluorescence quenching effect of gold nanoparticles. The results of cellular cytotoxicity and fluorescence confocal analyses showed that the PEG spacer-modified nanoparticles were essentially non-toxic and could be efficiently internalized in the cells within I hour of incubation. Intracellular particle tracking using a Keck 3-D Fusion Microscope System showed that the fanctionalized gold nanoparticles were rapidly internalized in the cells and localized in the peri-nuclear region. Using the PEG spacer, the gold nano-platform can be conjugated with a variety of biologically relevant ligands such as fluorescent dyes, antibodies, etc in order to target, probe, and induce a stimulus at the target site.
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