期刊
MOLECULAR IMAGING AND BIOLOGY
卷 8, 期 1, 页码 16-23出版社
SPRINGER
DOI: 10.1007/s11307-005-0025-0
关键词
sodium iodide symporter; isotopes; measles virus; virotherapy; oncolysis; molecular imaging; positron emission tomography; computerized tomography; gamma camera
资金
- NCI NIH HHS [CA 91956, CA 83181] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [R01CA083181, P50CA091956] Funding Source: NIH RePORTER
Purpose: With the advent of replication competent viruses for cancer gene therapy, it has become imperative to monitor the biodistribution, expression and replication of these vectors in living organisms. We evaluated the potential of 1-124 positron emission tomography (PET)/computed tomography (CT) imaging in gene therapy animal models utilizing the sodium iodide symporter (NIS) and compared the findings to 1-123 gamma camera imaging. Procedures: CB17 SCID mice were implanted with myeloma cell lines expressing NIS or infected by MV-NIS given systemically. Mice were imaged by both gamma camera (1-123) and PET/CT (1-124) and image quality assessed. Results: NIS expressing tumors concentrated 7.1% of the injected activity while tumors infected with the control virus had only 0.3% of the activity injected. Conclusions: 1-124 PET/CT in combination with NIS allows the tracking of stably transfected tumors or intravenously transfected tumors. Combined modality imaging using PET/CT allows accurate and non-invasive imaging of the distribution and gene expression of a replicating viral vector in living systems.
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