期刊
ACS CHEMICAL BIOLOGY
卷 10, 期 7, 页码 1648-1653出版社
AMER CHEMICAL SOC
DOI: 10.1021/acschembio.5b00230
关键词
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资金
- Basic Science Research Programs [2012R1A2A2A02016803, 2012R1A1A10017878, NRF-2009-0093826]
- Pioneer Research Center Program through the National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning [2014M3C1A3051460]
- Ministry of Education
- Ajou University research fund
The biological protein synthesis system has been engineered to incorporate unnatural amino acid into proteins, and this has opened up new routes for engineering proteins with novel compositions. While such systems have been successfully applied in research, there remains a need to develop new approaches with respect to the wider application of unnatural amino acids. In this study, we reported a strategy for incorporating unnatural amino acids into proteins by reassigning one of the Arg sense codons, the AGG codon. Using this method, several unnatural amino acids were quantitatively incorporated into the AGG site. Furthermore, we applied the method to multiple AGG sites, and even to tandem AGG sequences. The method developed and described here could be used for engineering proteins with diverse unnatural amino acids, particularly when employed in combination with other methods.
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