期刊
NUCLEIC ACIDS RESEARCH
卷 34, 期 11, 页码 3370-3377出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkl422
关键词
-
资金
- NATIONAL CANCER INSTITUTE [R01CA089562] Funding Source: NIH RePORTER
- NCI NIH HHS [CA 89562, R01 CA089562] Funding Source: Medline
The hybridization kinetics for a series of designed 25mer probe-target pairs having varying degrees of secondary structure have been measured by UV absorbance and surface plasmon resonance (SPR) spectroscopy in solution and on the surface, respectively. Kinetic rate constants derived from the resultant data decrease with increasing probe and target secondary structure similarly in both solution and surface environments. Specifically, addition of three intramolecular base pairs in the probe and target structure slow hybridization by a factor of two. For individual strands containing four or more intramolecular base pairs, hybridization cannot be described by a traditional two-state model in solution-phase nor on the surface. Surface hybridization rates are also 20- to 40-fold slower than solution-phase rates for identical sequences and conditions. These quantitative findings may have implications for the design of better biosensors, particularly those using probes with deliberate secondary structure.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据