期刊
NUCLEIC ACIDS RESEARCH
卷 34, 期 3, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gnj019
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资金
- Biotechnology and Biological Sciences Research Council [BBS/B/11400] Funding Source: Medline
We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage phi C31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or PgI in S. coelicolor. Cre-phage was also used for marker deletion in other strains of S. coelicolor.
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