期刊
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
卷 40, 期 1, 页码 76-86出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.yjmcc.2005.08.002
关键词
gene expression; microarray; cardiomyopathy; transcriptome; tachycardia-induced heart failure; RT-PCR; gene ontology; remodeling; pacing
资金
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL072488, P01HL077180] Funding Source: NIH RePORTER
- NHLBI NIH HHS [R01HL72488, R01 HL072488, R01 HL072488-02, P01 HL077180, P01HL077180] Funding Source: Medline
Alterations of cardiac gene expression are central to ventricular dysfunction ill human heart failure (HF). The canine tachycardia pacing-induced HF model is known to reproduce the main hemodynamic. echocardiographic and electrophysiological changes observed in human HE In this study, we use this HF model to compare gene expression profiles in the left and right ventricles (LV, RV) of normal and end-stage failing canine hearts and compare the transcription profiles to those in human and murine models of HE In end-stage HE the LV exhibits down regulation of genes involved in energy production, cardiac contraction, and modulation of excitation-contraction Coupling as compared with normal LV. The majority of transcriptomic changes between normal and end-stage canine HE were shared by the RV and LV. Genes down regulated only in the LV included those involved in aerobic energy production pathways, regulation of actin filament length, enzyme-linked receptor protein signaling pathways. In normal canine hearts. genes encoding specific components of the contractile apparatus exhibit LV-RV asymmetric expression patterns in failing hearts, cardiac fetal transcription factors MEF2 and MITF and the stress-responsive transcription factor ATF4 showed interventricular differences in expression. The comparison among the canine tachypacing, Mouse transgenic, and human HF reveals that human disease involves down regulation of genes in a broad range of biological processes while experimentally induced HF is associated with down regulation of energy pathways, and that human ischemic HF and canine HF share I similar over representation of transcriptional pathways in the LIP regulated genes. This study provides insights into the molecular pathways leading to end-stage tachycardia-induced HE and into global transcriptomic differences between the animal HF models and human HE (c) 2005 Published by Elsevier Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据