期刊
NEUROSCIENCE
卷 140, 期 3, 页码 1003-1010出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2006.02.070
关键词
astrocytes; chronic pain; glia; glial fibrillary acidic protein; mechanical allodynia
资金
- NATIONAL INSTITUTE ON DRUG ABUSE [R01DA011276] Funding Source: NIH RePORTER
- NIDA NIH HHS [DA11276] Funding Source: Medline
S100 beta is a calcium-binding peptide produced mainly by astrocytes that exerts paracrine and autocrine effects on neurons and glia. We have previously shown that S100 beta is markedly elevated at the mRNA level in the spinal cord following peripheral inflammation, intraplantar administration of complete Freund's adjuvant in the rat. The purpose of the present study was to further investigate the role of astrocytic S100 beta in mediating behavioral hypersensitivity in rodent models of persistent pain. First, we assessed the lumbar spinal cord expression of S100 beta at the mRNA and protein level using real-time RT-PCR, Western blot and immunohistochemistry analysis following L5 spinal nerve transection in rats, a rodent model of neuropathic pain. Second, we assessed behavioral hypersensitivity (mechanical allodynia) in wild type and genetically modified mice lacking or overexpressing S100 beta following L5 spinal nerve transection. Third, we assessed the expression level of S100 beta protein in the CD1 wild type mice after nerve injury. We report that lumbar spinal S100 beta mRNA steadily increased from days 4-28 after nerve injury. S100 beta protein in the lumbar spinal cord was significantly increased in both rats and mice at day 14 following nerve injury as compared with sham control groups. S100 beta genetically deficient mice displayed significantly increased tactile thresholds (reduced response to non-noxious stimuli) after nerve injury as compared with the wild type group. S100 beta overexpressing mice displayed significantly decreased tactile threshold responses (enhanced response to non-noxious stimuli). Together, these results from both series of experiments using a peripheral nerve injury model in two different species implicate the involvement of glial-derived S100 beta in the pathophysiology of neuropathic pain. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.
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