期刊
ORGANIC & BIOMOLECULAR CHEMISTRY
卷 4, 期 10, 页码 1966-1976出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b514748d
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The appearance of anionic phosphatidylserine ( PS) in the outer monolayer of the plasma membrane is a universal indicator of the early/intermediate stages of cell apoptosis. The most common method of detecting PS on a cell surface is to use the protein annexin V; however, in certain applications there is a need for alternative reagents. Recent research indicates that rationally designed zinc 2,2'-dipicolylamine (Zn2+ - DPA) coordination complexes can mimic the apoptosis sensing function of annexin V. Here, a series of. uorescently-labelled, tri- and pentapeptides with side chains containing Zn2+ - DPA are prepared and shown to selectively bind to anionic vesicle membranes. Fluorescein-labelled versions of the peptides are used to detect apoptotic cells by fluorescence microscopy and flow cytometry.
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