期刊
GENETICS
卷 172, 期 1, 页码 53-65出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.105.046441
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资金
- NATIONAL CANCER INSTITUTE [R01CA099036] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [F31GM065070, R01GM040334] Funding Source: NIH RePORTER
- NCI NIH HHS [R01 CA099036, R01 CA 99036] Funding Source: Medline
- NIGMS NIH HHS [F31 GM 65070, R01 GM040334, R01 GM40334, F31 GM065070] Funding Source: Medline
The spindle assembly checkpoint regulates the metaphase-to-anaphase transition from yeast to humans. We examined the genetic interactions with four spindle assembly checkpoint genes to identify nonessential genes involved in chromosome segregation, to identify the individual roles of the spindle assembly checkpoint genes within the checkpoint, and to reveal potential complexity that may exist. We used synthetic genetic array (SGA) analysis using spindle assembly checkpoint mutants mad1, mad2, mad3, and bub3. We found 228 synthetic interactions with the four spindle assembly checkpoint Mutants with substantial overlap in the spectrum of interactions between mad1, mad2, and W3. In contrast, there were many synthetic interactions that were common to mad1, mad2, and W3 that were not shared by mad3. We found shared interactions between pairs of spindle assembly checkpoint mutants, suggesting additional complexity within the checkpoint and unique interactions for all of the spindle assembly checkpoint genes. We show that most genes in the interaction network, including ones with unique interactions, affect chromosome transmission or microtubule function, suggesting that the complexity of interactions reflects diverse roles for the checkpoint genes within the checkpoint. Our analysis expands our understanding of the spindle assembly checkpoint and identifies new candidate genes with possible roles in chromosome transmission and mitotic spindle function.
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