期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 384, 期 1, 页码 191-198出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-005-0089-3
关键词
SELEX; microfluidic; miniaturized; aptamer; prototype
资金
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [S06GM008194] Funding Source: NIH RePORTER
- NIGMS NIH HHS [3S06GM 008194-25S1] Funding Source: Medline
Aptamers are nucleic acid binding species capable of recognizing a wide variety of targets ranging from small organic molecules to supramolecular structures, including organisms. They are isolated from combinatorial libraries of synthetic nucleic acid by an iterative process referred to as SELEX (Systematic Evolution of Ligands by Exponential Enrichment). Here we describe an automated microfluidic, microline-based assembly that uses LabView-controlled actuatable valves and a PCR machine, and which is capable of the selection and synthesis of an anti-lysozyme aptamer as verified by sequence analysis. The microfluidic prototype described is 1) a simple apparatus that is relatively inexpensive to assemble, making automated aptamer selection accessible to many investigators, and 2) useful for the continued morphing of macro -> meso -> microfabricated structures until a convergence to a few functional systems evolves and emerges, partly or completely achieving simpler, smaller and more rapid SELEX applications.
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