期刊
PROTEOMICS
卷 6, 期 1, 页码 72-80出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200402084
关键词
Mim1; mitochondria; outer membrane; proteome
资金
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM063883] Funding Source: NIH RePORTER
- NIGMS NIH HHS [GM 63883] Funding Source: Medline
The mitochondrial outer membrane mediates numerous interactions between the metabolic and genetic systems of mitochondria and the rest of the eukaryotic cell. We performed a proteomic study to discover novel functions of components of the mitochondrial outer membrane. Proteins of highly pure outer membrane vesicles (OMV) from Neurospora crassa were identified by a combination of LC-MS/MS of tryptic peptide digests and gel electrophoresis of solubilized OMV proteins, followed by their identification using MALDI-MS PMF. Among the 30 proteins found in at least three of four separate analyses were 23 proteins with known functions in the outer membrane. These included components of the import machinery (the TOM and TOB complexes), a pore-forming component (porin), and proteins that control fusion and fission of the organelle. in addition, proteins playing a role in various biosynthetic pathways, whose intracellular location had not been established previously, could be localized to the mitochondrial outer membrane. Thus, the proteome of the outer membrane can help in identifying new mitochondria-related functions.
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