期刊
MOLECULAR MICROBIOLOGY
卷 60, 期 6, 页码 1329-1343出版社
WILEY
DOI: 10.1111/j.1365-2958.2006.05177.x
关键词
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资金
- Intramural NIH HHS Funding Source: Medline
- NIAID NIH HHS [R01 AI024424, R37 AI024424, AI24424] Funding Source: Medline
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R37AI024424, R01AI024424, Z01AI000492] Funding Source: NIH RePORTER
The relapsing fever agent Borrelia hermsii undergoes multiphasic antigenic variation through gene conversion of a unique expression site on a linear plasmid by an archived variable antigen gene. To further characterize this mechanism we assessed the repertoire and organization of archived variable antigen genes by sequencing similar to 85% of plasmids bearing these genes. Most archived genes shared with the expressed gene a <= 62 nucleotide (nt) region, the upstream homology sequence (UHS), that surrounded the start codon. The 59 archived variable antigen genes were arrayed in clusters with 13 repetitive, 214 nt long downstream homology sequence (DHS) elements distributed among them. A fourteenth DHS element was downstream of the expression locus. Informative nucleotide polymorphisms in UHS regions and DHS elements were applied to the analysis of the expression site of relapse serotypes from 60 infected mice in a prospective study. For most recombinations, the upstream crossover occurred in the UHS's second half, and the downstream crossover was in the DHS's second half. Usually the closest archival DHS element was used, but occasionally a more distant DHS was employed. The downstream extragenic crossover site in B. hermsii contrasts with the downstream extragenic crossover site for antigenic variation in African trypanosomes.
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