期刊
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
卷 85, 期 2, 页码 491-494出版社
SPRINGER
DOI: 10.1007/s10973-006-7696-x
关键词
cadmium ion; copper ion; DNA; Spirulina platensis
Equilibrium dialysis and atomic absorption analysis were used to obtain adsorption isotherms and determine the stoichiometric binding constants of Cu(II) and Cd(II) ions to DNA from Spirulina platensis in solutions. The stoichiometric constants of Cu(II) and Cd(II) ions with DNA from S. platensis in 3 mM NaCl are 15.56(.)10(4) and 14.40(.)10(4), respectively. Effect of ionic strength and DNA GC content on binding constants of Cu(II)- and Cd(II)-DNA complexes were studied out. It was showed that the binding constants of Cu(II)- and Cd(II)-DNA complexes decrease with increase of ionic strength. The empirical dependences of logK on the GC content has been derived for Cd(II)- and Cu(II)-DNA complexes.
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