期刊
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
卷 56, 期 -, 页码 243-249出版社
MICROBIOLOGY SOC
DOI: 10.1099/ijs.0.63931-0
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Two novel species belonging to the genus Shewanella are described on the basis of their phenotypic characteristics, phylogenetic analyses of 16S rRNA and gyrB gene sequences and levels of DNA-DNA hybridization. A total of 47 strains belonging to two novel Gram-negative, psychrotolerant, H2S-producing bacterial species were isolated from marine fish (cod and flounder) caught from the Baltic Sea off Denmark. The phenotypic characteristics of strains belonging to group 1 (14 strains) indicated that these represented a non-sucrose-assimilating variant of Shewanella baltica with a DNA G + C content of 47.0 mol%. Strains of group 2 (33 isolates) did not utilize the carbon substrates assimilated by S. baltica except gluconate, N-acetylglucosamine and malate. Their DNA G + C content was 44.0 mol%. Phylogenetic analysis of the 16S rRNA gene sequence data placed the two novel species within the genus Shewanella. Group 1 strains showed greatest sequence similarity to Shewanella putrefaciens ATCC 8071(T) (99.0%) and with S. baltica NCTC 10375(T) (98.3%). However, gyrB gene sequence analysis showed these isolates to share only 90.0% sequence similarity with S. putrefaciens ATCC 8071(T) and 93.9% with S. baltica NCTC 10375(T). Similarly, DNA-DNA hybridization experiments revealed DNA relatedness levels of 38% between the group 1 isolates and S. putrefaciens ATCC 8071(T) and 43% with S. baltica NCTC 10375(T). The group 2 strains shared less than 97% 16S rRNA gene sequence similarities with recognized Shewanella species. Comparisons between the two novel species indicated 16S rRNA gene sequence similarity of similar to 98%, gyrB gene sequence similarity of similar to 89% and DNA-DNA reassociation values of 20-34%. Based on the evidence presented, two novel species, Shewanella hafniensis sp. nov. (type strain P010(T) = ATCC BAA-1207(T) = NBRC 100975(T)) and Shewanella morhuae sp. nov. (type strain U 1417(T) = ATCC BAA-1205(T) = NBRC 100978(T)), are described.
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