期刊
ANALYTICAL BIOCHEMISTRY
卷 348, 期 1, 页码 123-126出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2005.10.024
关键词
molecular beacon; site-specific DNA nickase; rolling-circle amplification
We have developed a simple isothermal (55 degrees C) reaction that permits detection of DNA targets using only two components: a molecular beacon and a site-specific DNA nickase without deoxyribonucleotide triphosphates and primers. The loop sequence of the molecular beacon should contain a DNA nickase recognition site. The nickase-molecular beacon (NMB) combination permits a 100-fold increase in fluorescent signal. The applications of the NMB assay for enhancement of fluorescent signal in some isothermal methods are discussed. (c) 2005 Elsevier Inc. All rights reserved.
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