期刊
MAGNETIC RESONANCE IN MEDICINE
卷 55, 期 6, 页码 1257-1264出版社
JOHN WILEY & SONS INC
DOI: 10.1002/mrm.20909
关键词
relaxation times; Lorentzian-Gaussian peak fitting; concentration; degradation; rotors
资金
- NCI NIH HHS [R01 CA59897, K01 CA96618] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [R01CA059897, K01CA096618] Funding Source: NIH RePORTER
A method was developed to quantify prostate metabolite concentrations using H-1 high-resolution magic angle spinning (HR-MAS) spectroscopy. T-1 and T-2 relaxation times (in milliseconds) were determined for the major prostate metabolites and an internal TSP standard, and used to optimize the acquisition and repetition times (TRs) at 11.7 T. At 1 degrees C, polyamines (PAs; T1(mean) = 100 +/- 13, T-2mean 30.8 +/- 7.4) and citrate (Cit; T1(mean) = 237 +/- 39, T2(mean) = 68.1 +/- 8.2) demonstrated the shortest relaxation times, while taurine (Tau; T1(mean) 636 +/- 78 T2(mean) 331 +/- 71) and choline (Cho; T1(mean) = 608 +/- 60, T2(mean) = 393 +/- 81) demonstrated the longest relaxation times. Millimolal metabolite concentrations were calculated for 60 postsurgical tissues using metabolite and TSP peak areas, and the mass of tissue and TSP. Phosphocholine plus glycerophosphocholine (PC+GPC), total choline (tCho), lactate (Lac), and alanine (Ala) concentrations were higher in prostate cancer ([PC+GPC](mean) = 9.34 +/- 6.4% [tCho](mean) = 13.8 +/- 7.41 [Lac](mean) = 69.8 +/- 27.1, [Ala](mean) 12.6 +/- 6.8) than in healthy glandular ([PC+GPC](mean) = 3.55 +/- 1.53, P < 0.01; [tChO](mean) = 7.06 +/- 2.36, P < 0.01; [LaC](mean) = 46.5 +/- 17.4, P < 0.01; [Ala](mean) 8.63 +/- 4.91, P = 0.051) and healthy stromal tissues ([PC+GPC](mean) = 4.34 +/- 2.46, P < 0.01; [tChO](mean) = 7.04 +/- 3.10, P < 0.01; [LaC](mean) = 45.1 +/- 18.6, P < 0.01; [Ala](mean) 6.80 +/- 2.95, P < 0.01), while Cit and PA concentrations were significantly higher in healthy glandular tissues ([Cit](mean) = 43.1 +/- 21.2 [PAs](mean) = 18.5 +/- 15.6) than in healthy stromal ([Cit](mean) = 16.1 +/- 5.6, P < 0.01; [PAs](mean) = 3.15 +/- 1.81, P < 0.01) and prostate cancer tissues ([Cit](mean) = 19.6 +/- 12.7, P < 0.01; [PAs](mean) = 5.28 +/- 5.44, P < 0.01). Serial spectra acquired over 12 hr indicated that the degradation of Cho-containing metabolites was minimized by acquiring HR-MAS data at VC compared to 20 degrees C.
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