期刊
NATURE PROTOCOLS
卷 1, 期 3, 页码 1145-1153出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2006.173
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资金
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT [R01HD052115] Funding Source: NIH RePORTER
- NATIONAL CANCER INSTITUTE [P30CA008748] Funding Source: NIH RePORTER
- NCI NIH HHS [P30 CA008748] Funding Source: Medline
- NICHD NIH HHS [R01 HD052115-01A1, R01 HD052115] Funding Source: Medline
The production of mouse chimeras is a common step in the establishment of genetically modified animal strains. Chimeras also provide a powerful experimental tool for following cell behavior during both prenatal and postnatal development. This protocol outlines a simple and economical technique for the production of large numbers of mouse chimeras using traditional diploid morula <-> diploid embryonic stem (ES) cell aggregations. Additional steps are included to describe the procedures necessary to produce specialized tetraploid chimeras using tetraploid morula <-> diploid ES cell aggregations. This increasingly popular form of chimera produces embryos of nearly complete ES cell derivation that can be used to speed transgenic production or ask developmental questions. Using this protocol, mouse chimeras can be generated and transferred to pseudopregnant surrogate mothers in a 5-d period.
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