期刊
DEVELOPMENTAL DYNAMICS
卷 235, 期 1, 页码 263-271出版社
WILEY
DOI: 10.1002/dvdy.20605
关键词
circadian rhythm; oscillation; Period1; Period2; Bmal1; cryptochrome1; cryptochrome2; mammary gland; development; differentiation; cell cycle
资金
- NATIONAL CANCER INSTITUTE [R01CA111551] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [P30ES009106] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [P01NS039546] Funding Source: NIH RePORTER
- NCI NIH HHS [R01 CA111551, R01CA111551, R01 CA111551-04] Funding Source: Medline
- NIEHS NIH HHS [P30ES09106, P30 ES009106] Funding Source: Medline
- NINDS NIH HHS [P01 NS39546, P01 NS039546] Funding Source: Medline
Mouse mammary epithelial cells (HC-11) and mammary tissues were analyzed for developmental changes in circadian clock, cellular proliferation, and differentiation marker genes. Expression of the clock genes Per1 and Bmal1 were elevated in differentiated HC-11 cells, whereas Per2 mRNA levels were higher in undifferentiated cells. This differentiation-dependent profile of clock gene expression was consistent with that observed in mouse mammary glands, as Per1 and Bmal1 mRNA levels were elevated in late pregnant and lactating mammary tissues, whereas Per2 expression was higher in proliferating virgin and early pregnant glands. In both HC-11 cells and mammary glands, elevated Per2 expression was positively correlated with c-Myc and Cyclin D1 mRNA levels, whereas Per1 and Bmal1 expression changed in conjunction with beta-casein mRNA levels. Interestingly, developmental stage had differential effects on rhythms of clock gene expression in the mammary gland. These data suggest that circadian clock genes may play a role in mouse mammary gland development and differentiation.
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