期刊
CANCER RESEARCH
卷 69, 期 4, 页码 1302-1313出版社
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/0008-5472.CAN-08-2741
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类别
资金
- NIH [CA66233, CA101860]
- University of Michigan Cancer Center [5 P 30 CA46592]
- INSERM
- Institut Paoli-Calmettes
- Taubman Institute
- Ministries of Health and Research
- Institut National du Cancer-Institut Lilly [AO 2006]
- Institut National du Cancer [PL2005, ACI2007]
- Ligue Nationale Contre le Cancer
Tumors may be initiated and maintained by a cellular subcomponent that displays stem cell properties. We have used the expression of aldehyde dehydrogenase as assessed by the ALDEFLUOR assay to isolate and characterize cancer stem cell (CSC) populations in 33 cell lines derived from normal and malignant mammary tissue. Twenty-three of the 33 cell lines contained an ALDEFLUOR-positive population that displayed stem cell properties in vitro and in NOD/SCID xenografts. Gene expression profiling identified a 413-gene CSC profile that included genes known to play a role in stem cell function, as well as genes such as CXCR1/IL-8RA not previously known to play such a role. Recombinant interleukin-8 (IL-8) increased mammosphere formation and the ALDEFLUOR-positive population in breast cancer cell lines. Finally, we show that ALDEFLUOR-positive cells are responsible for mediating metastasis. These studies confirm the hierarchical organization of immortalized cell lines, establish techniques that can facilitate the characterization of regulatory pathways of CSCs, and identify potential stem cell markers and therapeutic targets. [Cancer Res 2009;69(4):1302-13]
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