期刊
PAIN
卷 124, 期 1-2, 页码 222-233出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.pain.2006.05.028
关键词
trigeminal; demyelination; sodium channel; caspr; nodes of Ranvier
资金
- NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH [R01DE013942] Funding Source: NIH RePORTER
- NIDCR NIH HHS [DE 13942] Funding Source: Medline
The localization of sodium chatiriels NaChs) change following nerve lesions and this change may contribute to the development of increased pain states. Here we examine the change in distribution of NaChs within the rat infraorbital nerve (ION) two weeks after a combined inflammatory/partial axotomy lesion that results in behavior showing increased sensitivity to mechanical stimuli. Sections from experimental and normal control IONs were double-stained for indirect immunofluorescence using an antibody that identifies all NaCh isoforms and caspr-antibody to identify nodes of Ranvier, and a confocal microscope z-series of optically sectioned images were then obtained. ImageJ (NIH) software was used to quantify the area of pixels showing maximum NaCh intensity within both caspr and non-caspr associated accumulations. Analysis showed that the lesioned IONs had many more split nodes, heminodes and caspr-negative naked accumulations, a significantly increased area of NaCh staining within typical nodes and naked accumulations, as well as an increased density and size of significant accumulations when compared to normal IONs. This study demonstrates a dramatic redistribution and increased immunofluorescence of NaChs especially at myclinated and dernyelinated sites in fibers located just proximal to the lesion. The remodeling of NaChs seen in this study may represent an important event associated with the development of increased nerve excitability after lesions. (c) 2006 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.
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