期刊
CANCER RESEARCH
卷 69, 期 23, 页码 8862-8867出版社
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/0008-5472.CAN-09-1912
关键词
-
类别
资金
- Medical Research Council (MRC)
- Department of Health NIHR Biomedical Research Centres
- Institute of Child Health
- Child Health Research Appeal Trust
- A.S. Onassis Public Benefit Foundation
- Biotechnology and Biological Sciences Research Council
- British Heart Foundation
- Engineering and Physical Sciences Research Council
- King's College London
- UCL Comprehensive Cancer Imaging Centre Cancer Research UK
- Biotechnology and Biological Sciences Research Council [BB/D014727/1] Funding Source: researchfish
- Medical Research Council [G108/596, G0501781] Funding Source: researchfish
- BBSRC [BB/D014727/1] Funding Source: UKRI
- MRC [G108/596, G0501781] Funding Source: UKRI
The ability of mesenchymal stem cells (MSC) to specifically home to tumors has suggested their potential use as a delivery vehicle for cancer therapeutics. MSC integration into tumors has been shown in animal models using histopathologic techniques after animal sacrifice. Tracking the delivery and engraftment of MSCs into human tumors will need in vivo imaging techniques. We hypothesized that labeling MSCs with iron oxide nanoparticles would enable in vivo tracking with magnetic resonance imaging (MRI). Human MSCs were labeled in vitro with superparamagnetic iron oxide nanoparticles, with no effect on differentiation potential, proliferation, survival, or migration of the cells. In initial experiments, we showed that as few as 1,000 MSCs carrying iron oxide nanoparticles can be detected by MRI one month after their coinjection with breast cancer cells that formed subcutaneous tumors. Subsequently, we show that i.v.-injected iron-labeled MSCs could be tracked in vivo to multiple lung metastases using MRI, observations that were confirmed histologically. This is the first study to use MRI to track MSCs to lung metastases in vivo. This technique has the potential to show MSC integration into human tumors, allowing early-phase clinical studies examining MSC homing in patients with metastatic tumors. [Cancer Res 2009;69(23):8862-7]
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据