期刊
JOURNAL OF SURGICAL RESEARCH
卷 130, 期 1, 页码 58-65出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jss.2005.07.030
关键词
acute pancreatitis; Kupffer cells; NF-kappa B; AdI kappa B alpha; Fas/FasL; apoptosis; stress response; I kappa B alpha; TNF
类别
资金
- NIAAA NIH HHS [R24 AA12885] Funding Source: Medline
- NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM [R24AA012885] Funding Source: NIH RePORTER
Introduction. Nuclear factor (NF)-kappa B is a key transcriptional factor for cell survival, inflammation, and stress response. We demonstrated that Kupffer cell-derived FasL plays a central role in pancreatitis-induced hepatocyte injury. The aim of this study was to determine the role of NF-kappa B in regulating death ligand/receptor pathway in Kupffer cells during conditions that mimic acute pancreatitis. Materials and methods. Tissue cultures of rat Kupffer cells were treated with elastase (1 U/L) to mimic pancreatitis before and after infection with AdI kappa B to block activation of NF-kappa B. Tumor necrosis factor (enzyme-linked immunoassay), Fas/FasL, and caspase-3 (Western), tumor necrosis factor and Fas/FasL mRNA (reverse-transcription polymerase chain reaction), and NF-kappa B DNA binding (electrophoretic mobility shift assay) were determined. Apoptosis was measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) and DNA fragmentation. Gels were quantified by densitometry. Data (n = 3) are mean +/- SEM; student's t test was used for statistical analysis. Results. AdI kappa B infection up-regulated mutated I kappa B alpha that maintained its binding properties to NF-kappa B. Promoter-reporter assay demonstrated that FasL gene promoter was regulated by NF-kappa B. Infection with AdI kappa B attenuated the elastase-induced up-regulation of Fas/FasL (all P < 0.01 versus elastase) and NF-kappa B DNA binding but did not affect elastase-induced up-regulation of TNF. AdI kappa B attenuated elastase-induced cleavage of caspase-3, DNA fragmentation and TUNEL staining (all P < 0.01 versus elastase). Conclusions. Inhibition of NF-kappa B DNA binding down-regulates Fas/FasL and attenuates elastase-induced apoptosis; however, it has no effect on TNF production, suggesting that regulation of Fas/FasL and TNF may occur via different pathways. The ability of Kupffer cells to autoregulate their stress response by up-regulating their death ligand/receptor and apoptosis warrants further investigation. (c) 2006 Elsevier Inc. All rights reserved.
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