期刊
CLINICAL MICROBIOLOGY AND INFECTION
卷 13, 期 3, 页码 338-341出版社
BLACKWELL PUBLISHING
DOI: 10.1111/j.1469-0691.2006.01630.x
关键词
Anaplasma phagocytophilum; detection; Ehrlichia sp.; HF; Ixodes ricinus; PCR; ticks
DNA extracts from 156 tick pools, 18 blood specimens and 17 spleens from European woodmice (Apodemus sylvaticus) collected in Brittany, France were tested by PCR for the 16S rRNA gene of Anaplasmataceae. Positive amplicons were sequenced and confirmed, either by amplification and sequencing of a second gene, or by a second PCR specific for the P44 and gltA genes of Anaplasma phagocytophilum and the gltA gene of Ehrlichia sp. HF. In addition to A. phagocytophilum, the study detected Ehrlichia sp. HF for the first time in Ixodes ricinus ticks. This organism has only been detected previously in Ixodes ovatus ticks from Japan.
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