4.4 Article

Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs

期刊

JOURNAL OF VIROLOGICAL METHODS
卷 139, 期 1, 页码 39-43

出版社

ELSEVIER
DOI: 10.1016/j.jviromet.2006.09.003

关键词

polymerase chain reactions; pseudorabies virus; porcine cytomegalovirus; porcine circovirus

向作者/读者索取更多资源

The use of porcine organs is being developed as a means to alleviate the shortage of human organs for transplantation. Recommendations have been published for the microbiological specifications of organ-source pigs to reduce the possibility of a microorganism from pigs being inadvertently transferred to the recipient of the xenograft. The pseudorabies virus (PRV), porcine cytomegalovirus (PCMV), and porcine circovirus (PCV) are infectious agents in pigs that are considered to be of significance for the microbiological safety of xenotransplantation. A multiplex polyrmerase, chain reaction (mPCR) was developed to detect and differentiate among PRV, PCMV, and PCV. The sensitivities of the multiplex PCR were 10(2.5) TCID50/ml for PRV, 101 8 TCID50/ml for PCMV, and 10(1.8) TCID50/ml for PCV. The lowest viral concentrations detected by single PCR were 10(1.5) TCID50/ml for PRV, 10(1.0) TCID50/ml for PCMV, and 10(1.4) TCID50/ml for PCV2. Non-specific reactions were not observed when other viruses, bacteria, and Vero cells were used to assess the multiplex PCR. The multiplex PCR was effective in detecting various combinations of one or more of these viruses in pig specimens collected for xenotransplantation. (c) 2006 Elsevier B.V. All rights reserved.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据