期刊
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
卷 6, 期 9, 页码 1019-1026出版社
SPRINGERNATURE
DOI: 10.1039/b705101h
关键词
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Three porphyrin systems have been characterised for use in two-photon fluorescence imaging of biological samples. We have determined the two-photon absorption cross sections (sigma(2)) of the di-cation, free-base and metallated forms of hematoporphyrin derivative (HpD), hematoporphyrin IX ( Hp9) and a boronated protoporphyrin ( BOPP) using the open-aperture Z-scan and the two-photon induced fluorescence (TPIF) techniques at an excitation wavelength of 800 nm. The insertion of either protons or a metal ion into the macrocycle is shown not to significantly influence the sigma 2 of the porphyrins. Two-photon time-resolved fluorescence images of C6 glioma cells transfected with a free-base form of the BOPP have been obtained as a function of the porphyrin concentration. These studies reveal a maximum useful porphyrin concentration for fluorescence imaging purposes of approximately 30 mu g mL(-1).
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