期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 40, 期 2, 页码 187-192出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.lwt.2005.11.001
关键词
Escherichia coli O157 : H7; Spreeta (TM) biosensor; surface plasmon resonance; food safety
The Spreeta (TM), surface plasmon resonance (SPR)-based biosensor, was used to detect Escherichia coli O157:H7 spiked in milk, apple juice and ground beef extract using specific antibodies. In the Spreeta (TM) biosensor light from an LED is reflected off a gold surface, and the angle and intensity corresponding to the SPR minimum is measured and represented as a refractive index (RI) change corresponding to the antigen-antibody coupling at the sensor surface. Milk, apple juice, and ground beef patties spiked with E. coli O157:H7, at varying concentrations, were injected on the sensor surface immobilized with antibodies against the pathogen at a rate of 1 ml/min for a total of 2 min. The change in RI due to the binding of O157:H7 corresponding to each concentration was computed as an average of three replications over a 2 min interaction period. Assays were conducted at near real-time and results obtained after about 30 min of sample injection. Sensitivity of the E. coli O157:H7 assay was 10(2)-10(3) colony forming unit (CFU)/ml. The biosensor assay was also specific to E coli O157:H7 as other organisms (E coli K12 and Shigella) did not produce any appreciable change in the sensogram. Further experiments are needed to establish well-defined methods for detecting other food-borne pathogens in more complex and specific food matrices. (c) 2005 Swiss Society of Food Science and Technology. Published by Elsevier Ltd. All rights reserved.
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