Parathyroid-specific double knockout of G(q) and G(11) alpha-subunits leads to a phenotype resembling germline knockout of the extracellular Ca2+-sensing receptor

Germline knockout of the extracellular Ca2+-sensing receptor (CaR) leads to a phenotype that includes severe hypercalcemia, hyperparathyroidism, relative hypocalciuria, skeletal abnormalities, retarded growth, and early postnatal death. To investigate the role of heterotrimeric G proteins in CaR signaling, we used cre/lox technology to delete the respective alpha-subunits of G(q) and G(11) selectively in parathyroid cells. Mice that were PTH-Cre(+/-); Gnaq(flox/flox); Gna11(-/-) (PTH-G alpha(q)/G(alpha 11)-double knockouts) were viable, but showed all the features of germline knockout of the CaR except hypocalcuria. Our results demonstrate the critical role of both G(q) and G(11) in mediating inhibition of PTH secretion by extracellular Ca2+.