期刊
MOLECULAR REPRODUCTION AND DEVELOPMENT
卷 74, 期 1, 页码 42-47出版社
WILEY
DOI: 10.1002/mrd.20576
关键词
in vitro embryo culture; vitrification; lambing
Fatty acid-free bovine serum albumin (BSA(FAF)) can be added to supplement medium used in the culture of sheep embryos. BSA(FAF) was able to support blastocyst and subsequent embryo development at rates equivalent to that of fetal calf serum (FCS)-supplemented medium when fresh embryos were transferred. Furthermore, culture with BSA(FAF) significantly increased development of vitrified blastocysts transferred into synchronized sheep. The addition of the glycosaminoglycan, hyaluronan (HA) to the culture medium in the third and fifth day also increased cryo-tolerance of blastocysts and in turn lambing rate was improved.
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