Antioxidant activity of brocchlin carboxylic acid and its methyl ester from Chrozophora brocchiana

The analogue of brevifolin carboxylic acid and its methyl ester were isolated and identified from the aqueous ethanolic extract of the leaves of Chrzophora brocchiana in addition to eight known compounds identified as gallic acid, methyl and ethyl gallate, ellagic acid, monomethoxy and methylenedioxy ellagic acid, apigenin 7-O-glucoside and luteolin 7-O-glucoside. The structures were determined primarily by ESI-MS and NMR spectroscopy. The assignment of NMR signals was preformed by means of H-1-H COSY, HMQC and HMBC experiments. The antioxidant activity of the new compounds was determined by checking the scavenging activity against DPPH free radical.