期刊
PLANT SCIENCE
卷 172, 期 1, 页码 158-163出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2006.08.002
关键词
Taxus chinensis; paclitaxel; plant cell suspension culture; adaptation to chitosan stress; secondary metabolism; cell permeability
stress by comparing with the unadapted cells. Without elicitor treatment, the paclitaxel yield of the chitosan-adapted cells was two-fold that of the unadapted cells. When both cell cultures were elicited by Ag+ and methyl jasmonate (MJ), the paclitaxel yield of the chitosan-adapted cells was 4.6- and 3.2-fold that: of the unadapted cells, respectively; the paclitaxel release ratio of the chitosan-adapted cells was 2.8- and 3.2-fold that of the unadapted cells, respectively. Furthermore, upon elicitor treatment, the activation of phenylalanine ammonia-lyase (PAL) activity, a common and important response of plant cells to biotic and abiotic stresses, as well as the cell viability and permeability of the chitosan-adapted cells was significantly higher, compared with the unadapted cell cultures, which corresponded well to the superior paclitaxel yield and release. These results suggest that adaptation to chitosan is an effective strategy for improving paclitaxel yield and release of T. chinensis cells. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
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