期刊
MICROBIOLOGY AND IMMUNOLOGY
卷 51, 期 5, 页码 547-550出版社
WILEY
DOI: 10.1111/j.1348-0421.2007.tb03932.x
关键词
NoV; RT-LAMP; simultaneous detection; genogrouping
We developed a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of noroviruses (NoVs) in a genogroup-specific manner in a previous study. In this study, to detect NoVs more easily and simply, we have developed an RT-LAMP assay for the simultaneous detection of NoV genogroup I (GI) and II (GII) genomes in a single tube. The genogrouping was achieved by using fluorescence-labeled primers, and the green and red colors for GI and GII, respectively, sometimes with yellow color for GI and GII mixture, were indicated on the agarose gel under UV light 312 nm.
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