期刊
GLYCOBIOLOGY
卷 17, 期 1, 页码 25-35出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwl046
关键词
cartilage; glycomics; glycosaminoglycan; mass spectrometry
资金
- NCRR NIH HHS [P41 RR10888, P41 RR010888, P41 RR010888-118066] Funding Source: Medline
- NHLBI NIH HHS [R01 HL074197-04, R01 HL074197, R01 HL74197] Funding Source: Medline
- NIA NIH HHS [R03 AG023307] Funding Source: Medline
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR010888] Funding Source: NIH RePORTER
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL074197] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R03AG023307] Funding Source: NIH RePORTER
Articular cartilage is a highly specialized smooth connective tissue whose proper functioning depends on the maintenance of an extracellular matrix consisting of an integrated assembly of collagens, glycoproteins, proteoglycans (PG), and glycosaminoglycans. Isomeric chondroitin sulfate glycoforms differing in position and degree of sulfation and uronic acid epimerization play specific and distinct functional roles during development and disease onset. This work introduces a novel glycosaminoglycan extraction method for the quantification of mixtures of chondroitin sulfate oligosaccharides from intact cartilage tissue for mass spectral analysis. Glycosaminoglycans were extracted from intact cartilage samples using a combination of ethanol precipitation and enzymatic release followed by reversed-phase and strong anion exchange solid-phase extraction steps. Extracted chondroitin sulfate glycosaminoglycans were partially depolymerized using chondroitinases, labeled with 2-anthranilic acid-d(4) (2-AA) and subjected to size exclusion chromatography with online electrospray ionization mass spectrometric detection in the negative ion mode. The method presented herein enabled simultaneous determination of sulfate position and uronic acid epimerization in juvenile bovine and adult human cartilage samples. The method was applied to a series of 13 adult human cartilage explants. Standard deviation of the mean for the measurements was 1.6 on average. Coefficients of variation were approximately 4% for all compositions of 40% or greater. These results show that the new method has sufficient accuracy to allow determination of topographical distribution of glycoforms in connective tissue.
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