期刊
PLANT SIGNALING & BEHAVIOR
卷 2, 期 5, 页码 416-420出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/psb.2.5.4416
关键词
protein microarray; calmodulin; protein-protein interactions; large scale protein production
资金
- Yale University Genomics and Proteomics Center
- NSF [MCB-0313514, DBI-0519853]
Protein microarray technology has emerged as a powerful new approach for the study of thousands of proteins simultaneously. Protein microarrays have been used for a wide variety of applications for the human and yeast systems. In a recent study, we demonstrated that Arabidopsis functional protein microarrays can be generated and employed to characterize the function of plant proteins. The arrayed proteins were produced using an optimized large-scale plant-based expression system. In a proof-of concept study, 173 known and novel potential substrates of calmodulin (CaM) and calmodulin-like proteins (CML) were identified in an unbiased and high-throughput manner. The information documented here on novel potential CaM targets provides new testable hypotheses in the area of CaM/Ca2+-regulated processes and represents a resource of functional information for the scientific community.
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