期刊
EXPERT OPINION ON DRUG DISCOVERY
卷 2, 期 4, 页码 571-589出版社
TAYLOR & FRANCIS LTD
DOI: 10.1517/17460441.2.4.571
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The Gateway cloning system sets a new trend in molecular biology by addressing the difficulties of adaptability, efficiency and compatibility of the traditional cloning approaches. Based on the well-characterized site-specific recombination system of phage lambda, the Gateway technology allows the cloning, combining and transferring of DNA segments between different expression platforms in a high-throughput manner while maintaining orientation and the reading frame of the fragment or fragments of interest. In this article, the key-aspects and potential applications of this system are reviewed. Benefits and weaknesses of this and other cloning technologies are discussed.
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